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CRISPRgenee: New Method Boosts Genetic Loss-of-Function Analyses

Say goodbye to hard-to-target genes. This new method lets scientists study cellular processes more effectively than ever before.

It is an edited image of flowers and plants.
It is an edited image of flowers and plants.

CRISPRgenee: New Method Boosts Genetic Loss-of-Function Analyses

Researchers at the University of Stuttgart have made a significant breakthrough in genetic loss-of-function (LOF) analyses. Led by Dr. Phillip Rathert, the team has developed CRISPRgenee, a novel CRISPR method that combines silencing and cutting a target gene simultaneously, making LOF analyses more efficient.

The Rathert group's work focuses on understanding how cells control their genes to maintain a healthy state. Their new method, CRISPRgenee, builds upon the 2020 Nobel Prize-winning CRISPR/Cas9 technology. Unlike conventional methods, CRISPRgenee can effectively switch off genes previously difficult to target. Additionally, it allows for the analysis of two different genes at once, providing a more comprehensive understanding of cellular functions.

The team tested CRISPRgenee in various biological systems, including cell proliferation, epithelial-mesenchymal transition, and neuronal differentiation in human induced pluripotent stem cells (iPS cells). The results, published on September 22, 2025, in Cell Reports Methods, demonstrate the method's versatility and potential. The study was a collaboration between the University of Stuttgart and other institutes.

CRISPRgenee, developed by Dr. Phillip Rathert's team at the University of Stuttgart, offers a more efficient and versatile tool for genetic loss-of-function analyses. By combining silencing and cutting, it opens new avenues for understanding cellular processes and maintaining health. The method's potential was demonstrated in various biological systems and was published in Cell Reports Methods.

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